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The cell line was cultured in basal bronchial epithelial cell basal medium (BEBM) (Lonza, Walkersville, MD) supplemented with 2 ml of bovine pituitary extract and 0.5 ml of: insulin, hydrocortisone, retinoic acid, transferrin, triiodothyronine, epinephrine, and human recombinant epidermal growth factor (Lonza, Walkersville, MD)

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Collectively, these findings indicate that p53-mediated tumour suppression is a complex process and that p53 target genes other than Puma , Noxa and p21 and cellular processes in addition to apoptosis and cell cycle arrest/cell senescence must also be involved

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Venugopal PD, Morse AL, Alrefai-Kirkpatrick R, Tworek C, Chang HW